Coding

Part:BBa_K1962005:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-11)


Immunity Protein Im-E9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compliant with RFC[10].


Source

The primary protein sequence was obtained from Uniprot and then back-translated into DNA before being codon optimised for E. coli K-12. The resultant biobrick was synthesised in its entirety by IDT as a gBlock gene fragment.


References